Expression of hepatitis B surface antigen gene in yeast.
نویسندگان
چکیده
The DNA sequence coding for hepatitis B virus surface antigen (HBsAg) was placed under control of the repressible acid phosphatase promoter of the yeast Saccharomyces cerevisiae in a plasmid capable of autonomous replication in both yeast and Escherichia coli. Yeast transformed by this plasmid synthesized up to 5 X 10(5) molecules per cell of immunologically active HBsAg polypeptide in phosphate-free medium. The HBsAg polypeptides produced in the yeast cells were assembled into 20- to 22-nm spherical or oval particles and were immunogenic.
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عنوان ژورنال:
- Proceedings of the National Academy of Sciences of the United States of America
دوره 80 1 شماره
صفحات -
تاریخ انتشار 1983